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Here, we investigated the adjuvant mechanism of cationic liposomes based on the synthetic amphiphile dimethyldioctadecylammonium DDA. This efficient adsorption onto the liposomes led to an enhanced uptake of OVA by BM-DCs as assessed by flow cytometry and confocal fluorescence laser-scanning microscopy.

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DDA liposomes were prepared using the aqueous heat method. For incorporation of antigen, OVA was mixed with DDA liposomes in sterile distilled water and left for 1 hr at room temperature with intermittent mixing. The amount of OVA in supernatants and pellets was zctivation by sodium dodecyl sulphate—polyacrylamide gel electrophoresis SDS—PAGE followed by silver staining according to the protocols described by Laemmli 34 and Blum et al.

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