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DDA liposomes were prepared using the aqueous heat method. For incorporation of antigen, OVA was mixed with DDA liposomes in sterile distilled water and left for 1 hr at room temperature with intermittent mixing. The amount of OVA in supernatants and pellets was zctivation by sodium dodecyl sulphate—polyacrylamide gel electrophoresis SDS—PAGE followed by silver staining according to the protocols described by Laemmli 34 and Blum et al.

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